The primers are present in such a large excess that they &ldquo;out compete&rdquo; the parent strands coming back together

The 54&deg; C is not below sufficiently below the Tm for the strands to recombine

The DNA polymerase binds to the single strands and prevents them from coming back together to form a double helix.

During the polymerase chain reaction, the two DNA strand are separated at 95 °C after which the reaction mixture is cooled to 54 °C to allow the primers to hybridize to the DNA strands. Why do parent DNA duplexes not form instead?

During the polymerase chain reaction, the two DNA strand are separated at 95 &deg;C after which the reaction mixture is cooled to 54 &deg;C to allow the primers to hybridize to the DNA strands. Why do parent DNA duplexes not form instead?

Related Questions

M.Sc. Entrance

CSIR NET

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