Primary production in aquatic ecosystem is measured using Light-and-Dark-Bottle technique. In this method, as an indirect  measure of photosynthetic production, dissolved oxygen concentration of the pond water enclosed in a BOD bottle is  measured initially (I) and after a fixed duration of incubation in a light bottle (L) and a dark bottle (D) Then, the gross and net  primary productions are estimated is

<p>Primary production in the aquatic ecosystem is measured using the Light-and-Dark-Bottle technique. In this method, as an indirect measure of photosynthetic production, the dissolved oxygen concentration of the pond water enclosed in a BOD bottle is measured initially (I) and after a fixed duration of incubation in a light bottle (L) and a dark bottle (D) Then, the gross and net primary productions are estimated is</p>

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