Routine microscopy could not resolve whether the gene product is localized inside the nucleus or on the nuclear membrane

You wish to localize a given gene product at subcellular levels following immunofluorescene staining. Routine microscopy could not resolve whether the gene product is localized inside the nucleus or on the nuclear membrane.
Which of the following will resolve this unambiguously?
P. Sectioning of cell followed by phase contrast microscopy. Q. A simulation of 3D picture following confocal microscopy. R. Optical sectioning and observing each section. S. Freeze fracturing followed by Scanning Electron Microscopy

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