In resting cells, proteins X and Y are localized in the cytosol. Upon stimulation with lipopolysaccharide (LPS), both of them are phosphorylated and translocate to the nucleus. You have used antibodies against phosphorylated form of proteins X and Y which are conjugated to either red, or green or blue dye. Keeping optical aberration of lens in mind which one of the following will be the best for visualizing X and Y in the nucleus by fluorescence microscopy ?
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