Individual I is homozygous for the variant allele

Individual II is homozygous for variant allele

Individual II is homozygous for wild type allele

Individual III is homozygous for wild type allele

In 'TagMan' assay for detection of base substitutions (DNA variant), probes (oligonucleotides) with Fluorescent dyes at the 5'-end and a quencher at 3'-end are used. While the probe is intact, the proximity of the quencher reduces the Fluorescence emitted by reporter dye. If the target sequences (wild type or the variant) are present, the probe anneals to the target sequence, down stream to one of the primers used for amplifying the DNA sequence flanking the position of the variants. For an assay two flanking PCR primers, two probes corresponding to the wild type and variant allele and labelled with two different reporter dyes and quencher were used. During extension the probe may be cleaved by the Taq-polymerase separating the reporter dye and the quencher. Three individuals were genotyped using this assay. Sample for individual I shows maximum fluorescence for the dye attached to the wild type probe, sample for individual II shows maximum fluorescence for the dye attached to variant probe and sample for individual III exhibits equal fluorescence for both the dyes. Which of the following statement is correct? 

In 'TagMan' assay for detection of base substitutions (DNA variant), probes (oligonucleotides) with Fluorescent dyes at the 5'-end and a quencher at 3'-end are used. While the probe is intact, the proximity of the quencher reduces the Fluorescence emitted by reporter dye. If the target sequences (wild type or the variant) are present, the probe anneals to the target sequence, down stream to one of the primers used for amplifying the DNA sequence flanking the position of the variants. For an assay two flanking PCR primers, two probes corresponding to the wild type and variant allele and labelled with two different reporter dyes and quencher were used. During extension the probe may be cleaved by the Taq-polymerase separating the reporter dye and the quencher. Three individuals were genotyped using this assay. Sample for individual I shows maximum fluorescence for the dye attached to the wild type probe, sample for individual II shows maximum fluorescence for the dye attached to variant probe and sample for individual III exhibits equal fluorescence for both the dyes. Which of the following statement is correct?&nbsp;

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M.Sc. Entrance

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