Proteins with similar isoelectric points become further separated according to their molecular weights

The individual bands become stained so that the isoelectric focus pattern can be visualized

The individual bands become visualized by interacting with protein-specificantibodies in the second gel

The individual bands undergo a second, more intense isoelectric focusing

The first step in two-dimensional gel electrophoresis generates a series of protein bands by isoelectric focusing. In a second step a strip of this gel is turned 90 degrees, placed on another gel containing SDS, and electric current is again applied. In this second step :

The first step in two-dimensional gel electrophoresis generates a series of protein bands by isoelectric focusing. In a second step a strip of this gel is turned 90 degrees, placed on another gel containing SDS, and electric current is again applied. In this second step :

Related Questions

M.Sc. Entrance

CSIR NET

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