As a DNA is linear, the number of restriction sites for each enzyme is Xbal 2, Xhol 2, Kpnl

The Xbal and Xhol sites can be mapped&nbsp;

All the Kpnl sites fall in the 24.5 kb Xbal fragment, as the 24.0 kb fragment is intact after Xbal-Kpnl double digestion. The order of the Kpnl fragments can be determined only by partial digestion&nbsp;

Total genome size of phage is 48.5 kb

To construct a restriction map, a series of restriction digests must be performed First, the number and sizes of the fragments produced by each restriction endonuclease must be determined by gel electrophoresis followed by comparison with size markers (Figure) this information must then he supplemented by a series of double digestions,

To construct a restriction map, a series of restriction digests must be performed First, the number and sizes of the fragments produced by each restriction endonuclease must be determined by gel electrophoresis followed by comparison with size markers (Figure) this information must then he supplemented by a series of double digestions, in which the DNA is cut by two restriction endonucleases at once. It might be possible to perform a double digestion in one step if both enzymes have similar requirements for PH, Mg2+ concentration, etc. Alternatively, the two digestions may have to be carried out one after the other, adjusting the reaction mixture after the first digestion to provide a different set of conditions for the second enzyme. Comparing the results of single and double digests will allow many, if not all, of the restriction sites to be mapped. Results obtained during single and double digestion of lambda phage with restriction enzymes are summarized in table below
<img src="https://vediphlie-aws.s3.ap-south-1.amazonaws.com/27_65d8c1456f.JPG" alt="" />
Enzymes&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;Number of fragments&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Sizes (kb)
Xbal&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;2&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;24.0, 24.5
Xhol&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; 2&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; 15.0, 33.3
Kpnl&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; 3&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; 1.5, 17.0 30.0
Xbal + Xhol&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; 3&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;9.0, 15.0, 24.5
Xbal + Kpnl&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; 4&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;1.5, 6.0, 17.0, 14.0
Which of the following inference in not correct?

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